Polypeptide fraction affording protection to cells against mechanical damage and assay

Fraction polypeptidique conferant une protection a des cellules contre les dommages mecaniques et les dosages

Abstract

Abstract of the Disclosure A cell protective composition comprises a polypeptide fraction capable of inhibiting mechanical damage to cells and heat-stable up to about 100°C, inhibitory of mechanical cell damage at about pH 5.5 to 7.5, inhibitory activity unaffected by ribonuclease and/or deoxyribonuclease enzymes but partially lost to trypsin, molecular weight about 6-100 Kdaltons, denatured by ethanol precipitation, acid labile at pH below about 1.2, and trichloroacetic acid-precipitable and protectively active upon redissolution. The polypeptide fraction may be prepared by separating plasma from blood, heating the plasma to about 90 to 100°C to form a heat-labile precipitate and a heat-stable supernate, separating the heat-labile precipitate from the heat-stable supernate, and separating a polypeptide fraction of molecular weight about 6 to 100 Kdaltons from the heat-stable supernate. An in vitro method of assessing the degree of protection from mechanical damage afforded cells in a biological sample, comprises placing cells in a medium under conditions effective for growth, adding to the medium particles capable of undergoing a mild mechanical interaction with the cells, adding to the medium a substance to be tested, agitating the medium comprising the cells for a preset period of time under preset conditions, and determining the amount in the medium of a factor associated with the degree of mechanical damage of the cell.

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